Tag: solvents
What do I do if a 2-solvent gradient will not separate my sample?
Usually, a 2-solvent or binary gradient will separate your desired compound from the by-products and impurities. Sometimes though, you can encounter a mixture in which some compounds co-elute and are not separable with any binary gradient you try.
I encountered this situation recently while trying to purify a lavender essential oil and have dedicated this post to how I solved the problem. Continue reading What do I do if a 2-solvent gradient will not separate my sample?
How does mobile phase organic solvent choice impact reversed-phase flash column chromatography?
Organic and medicinal chemists frequently utilize flash chromatography to purify their reaction mixtures. Normal-phase flash chromatography is most often used but may not the best methodology, especially when the compounds are quite polar and/or ionizable.
For these molecules, reversed-phase flash chromatography is preferred but often is not used due to an uncertainty regarding the best solvent choices and the reversed-phase mechanism. In this post, I will discuss how organic solvent choice in reversed-phase chromatography can influence the chromatographic separation.
Why do I see more peaks than I expect with flash column chromatography?
Are you observing more chromatographic peaks than you expect compared to TLC or other assessment data? Well, it could be that your method is separating some isomers or, it could be that there is an actual method issue.
In this post I will discuss what could cause a method issue and suggest some ideas as to how to fix it. Continue reading Why do I see more peaks than I expect with flash column chromatography?
Using pH to optimize reversed-phase flash chromatography separations
I have previously posted on the topic of normal-phase optimization by evaluating different solvent blends or mixtures. I have also touched on reversed-phase method development as well suggesting chemists use HPLC to optimize their purification.
In this post, I will look at the impact modifying mobile phase pH can have on reversed-phase separations.
Continue reading Using pH to optimize reversed-phase flash chromatography separations
How do I remove an annoying MS TIC background?
Have you ever run flash column chromatography with mass detection (Flash-MS) and observed the total ion current or TIC increase during the purification only to find that there was no discernible compound contributing to the effect?
In this post I discuss how I came across this issue and the solution I found to work.
Continue reading How do I remove an annoying MS TIC background?
