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In my role as senior technical specialist at Biotage I am often asked about compound detection options. For most flash chromatography methods, UV is the default detection tool since a majority of compounds do absorb some UV light.
Diode array UV detectors provide chemists choices in wavelength selection, providing the ability to widen or narrow the wavelength range needed to detect specific compounds and enhance their sensitivity.
When diode array detectors fail to detect compounds, it is because the compounds have no chromophore, e.g. carbohydrates, low extinction coefficients, exist in really low concentrations, or any combination of these. In these situations, alternative detectors are quite beneficial. In this post I will discuss a couple of detector options for flash chromatography.
Continue reading So, which detector should I use for flash column chromatography?
The bane of organic synthesis for most chemists is purification rather than synthesis. Synthetic reaction mixtures are rarely devoid of impurities so some type of purification is necessary. Most often flash chromatography is used but for many chemists, it is less well understood than their chemical reaction and provides some level of anxiety.
In this post, I will summarize the five most important steps to creating a successful flash chromatography method and thus the anxiety associated with it.
Continue reading 5 Steps to successful flash chromatography
I have recently posted on how solvent choice influences the separation of hard to resolve compounds using normal-phase flash chromatography. As a chemist with an inquiring mind, I thought I would expand my research beyond normal-phase and see what happens in reversed-phase.
In this post, I share my results.
Continue reading How does solvent choice impact reversed-phase flash chromatography separations?