How do I remove an annoying MS TIC background?

Have you ever run flash column chromatography with mass detection (Flash-MS) and observed the total ion current or TIC increase during the purification only to find that there was no discernible compound contributing to the effect?

In this post I discuss how I came across this issue and the solution I found to work.

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Cannabis extract purification using orthogonal flash column chromatography

For some cannabis-based product developers reversed-phase chromatography has become the analytical tool of choice for determining the extract content profile as well as for purification of specific cannabinoid compounds. However, the extracts often contain many other compounds which reduce load capacity and purity of the product(s) of interest and then require even more extensive clean-up.

In this post I show the results of an orthogonal flash purification approach that first uses normal-phase flash column chromatography to clean up the crude cannabis extract followed by reversed-phase C18 flash chromatography of the isolated target compounds.  This orthogonal approach to purification increases the targeted product’s purity.

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