Flash chromatography – a purification tool for both organic chemists and natural product researchers. This tool is essential when you need to remove impurities from your targeted product, or products, in order to get them pure. To reduce the costs associated with flash chromatography, some chemists try reusing the same column over and over, not always with success.
A question I am frequently asked is “how many times can I reuse my flash column?” Although I have previously addressed this topic, I feel it is worth another look. In this post, I will attempt to address this question by providing a bit more science behind the cartridge reuse question.
The challenges organic, medicinal, and natural product chemists face are many: from designing reactions, to optimizing synthesis, work-up / extraction, and purification / isolation of the desired compound or compounds. Among those issues related to purification / isolation is the common problem of separating compounds with similar chemistry that either co-elute or separate poorly.
In this post I will discuss some tips on how to “resolve” this issue (yes, pun intended).
For most organic reaction mixture purifications the process is fairly straightforward. Use hexane/ethyl acetate or, for polar compounds, DCM/MeOH. But what do you do if this doesn’t work and your compounds are basic organic amines?
In this post, I re-examine the options available to achieve an acceptable organic amine purification when typical separation methods are insufficient. Continue reading How do I purify ionizable organic amine compounds using flash column chromatography?
Selectivity and solvent strength are the most important factors that determine success or failure of a chromatographic separation. These two independent dynamics apply to both normal- and reversed-phase chromatography and should be optimized, especially when high fraction purity is needed.
In this post I will discuss the impact that elution solvent choice has on both normal- and reversed-phase purification. Continue reading How does solvent choice impact flash column chromatography performance?
A question I hear a lot from chemists is “how much can I load”. The answer is always “it depends on your separation quality”. At that point I begin asking about the TLC data and purification goals. Purification goal setting should be your first step and the question to answer is – what do I need this purification to achieve? Is the goal high purity, high yield, or some combination. Remember, you will typically sacrifice purity for high yield and yield for high purity so optimization is an important consideration.
In this post I will discuss the results of a study I conducted where I continually increased the crude sample load until my target compound purity fell below specific targeted levels to understand the real loading limitation. Continue reading Pushing flash column chromatography loading limits
Got a Biotage® SNAP Ultra cartridge but no Isolera™ flash system? Don’t worry, the design of Biotage SNAP cartridges allows use on virtually any modern system for flash purification.
Here we go – the first step towards the ultimate flash purification online resource. Hope to see you soon!