All there is to know about flash purification – a blog by Biotage
How does solvent choice impact flash column chromatography performance?
Selectivity and solvent strength are the most important factors that determine success or failure of a chromatographic separation. These two independent dynamics apply to both normal- and reversed-phase chromatography and should be optimized, especially when high fraction purity is needed.
In this post I will discuss the impact that elution solvent choice has on both normal- and reversed-phase purification.