Item added to cart
Shopping Cart (Login required)
Sample Preparation Horizon Food & Environmental Products Organic Synthesis Peptide Chemistry Flash Purification Work-up Products Evaporation & Concentration Scale-Up Products Biomolecules Service & Support


SPE Columns and Plates

Biotage sample preparation column size table

ISOLUTE® HCX-5 is a mixed-mode sorbent providing clean extracts for basic compounds from complex biological fluid samples using a dual retention mechanism.

ISOLUTE® HCX-5 combines non-polar (C4) and strong cation exchange retention  (SO3-) mechanisms to isolate basic analytes from complex aqueous matrices such as urine and plasma.

Analytes are initially retained by a non-polar retention mechanism, with inorganic salts, polar interferences such as phospholipids and large molecular weight peptides and proteins passing directly through the sorbent bed. The pH is then adjusted to allow an ion exchange retention mechanism to retain the analyte(s). Non-polar interferences are removed using an organic solvent or organic solvent/water wash. Analytes are then eluted in an extra clean fraction.

ISOLUTE HCX-5 provides the cleanest extracts of all the ISOLUTE mixed-mode sorbents. It is the ideal choice where the analyte to be extracted has sufficient non-polar charater to be well retained by the non-polar (C4) component of the mixed-mode sorbent.

ISOLUTE HCX-5 is available in a wide range of SPE columns and 96-well plate formats.