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Advanced Polymeric SPE for Acidic Analytes

Solid Phase Extraction Evolute CX

This product has been replaced by the EVOLUTE® EXPRESS product line.

EVOLUTE® AX combines non-polar and strong anion exchange functionality for extraction of acidic analytes from biological fluids and other aqueous samples through a dual, mixed-mode mechanism.  This provides additional selectivity and clean-up for complex samples, with salts, phospholipids and proteins eliminated from extracts.   Sorbent performance is not compromised by drying during processing.  Available in a variety of formats, including modular and 96-well plates and SPE columns, and two different particle sizes (30 µm and 50 µm) depending on application requirements.

The sorbent consists of the EVOLUTE® backbone, surface modified with quaternary amine  groups.

Figure 1. Structure of EVOLUTE AX sorbent

EVOLUTE® AX sorbent is available in EVOLUTE® EXPRESS 96-well plate format for use with fast Load-Wash-Elute procedures.  Eliminate traditional conditioning and equilibration and increase throughput without compromising performance.

High Selectivity Provides Ultra-clean Extracts

EVOLUTE® sorbents have a narrow pore diameter, tailored to eliminate co-extraction of proteins from biofluids. Removal of proteins during sample preparation avoids transfer in to the analytical system, reducing matrix effects in LC-MS/MS, and avoiding contamination of the analytical column. This minimises the need for frequent backflushing and guard columns replacement.

Using EVOLUTE AX, interferences including proteins, salts, non-ionisable molecules and phospholipids are removed during the interference elution step, ensuring extremely clean extracts (figure 2).


Figure 2. The combination of optimized pore structure and additional interference elution steps using mixed-mode sorbent chemistry guarantees extremely clean extracts.

High Analyte Recoveries

EVOLUTE AX uses a generic procedure for extraction of acidic analytes in aqueous matrices. The dual retention mechanism retains bases through a combination of non-polar and strong anion exchange interactions. The simple wash steps in the generic method remove >98% of phospholipids from plasma samples.

The combination of robust polymer chemistry, uniform coverage of quaternary amine groups and optimum exchange capacity provide greater than 85 % recovery for a wide range of basic analytes.

EVOLUTE AX should not be used for extraction of sulfonic or other strong acids, (see EVOLUTE WAX).

 See the EVOLUTE User Guide for a wealth of method optimisation information.

 Excellent Flow Characteristics

Narrow particle size distribution provides consistent flow characteristics for a variety of sample matrices. Optimized packing techniques eliminate channelling, which can lead to analyte breakthrough. Additionally, larger particle EVOLUTE AX 50 µm allows for increased flow rates when using particularly viscous samples or large sample volumes.

Batch-to-batch reproducibility

The surface characteristics of each batch of EVOLUTE AX are quality controlled and tested to ensure consistency.

  • High, reproducible recoveries for basic analytes
  • Eliminate salts, phospholipids and proteins from biofluid extracts, and minimise ion suppression
  • Fast simple generic methodology
  • Available in EVOLUTE EXPRESS plates for fast  Load-Wash-Elute procedures