ISOLUTE® Solid Phase Extraction products are a range of leading quality SPE columns and 96-well plates, packed with a variety of ISOLUTE® non-polar, mixed-mode, polar and ion exchange sorbents. Quality controlled sorbent chemistry, particle size distribution, component purity and manufacturing procedures mean that ISOLUTE® SPE products perform reliably time after time. From biological fluids to wastewater samples, the wide range of sorbents and formats along with the wealth of technical and application support available from Biotage mean you can develop robust, reproducible SPE methods giving high analyte recovery and clean extracts.
Solid Phase Extraction (SPE) is a sample preparation technique used to isolate (purify) and/or concentrate (trace enrich) analytes. The range of possible analytes and matrices is vast. The ISOLUTE® range of SPE sorbents and formats offers the scientist the breadth of choice needed to develop an optimised solution to a multitude of challenging sample preparation problems.
ISOLUTE® sorbents are available packed into a range of columns and plate formats.
SPE columns are available with reservoir volumes ranging from 1 mL to 70 mL, with tabless options for automation compatibility, and options for high sensitivity analyses.
ISOLUTE® SPE products are manufactured to the highest quality standards. State of the art manufacturing techniques are supported by a comprehensive Quality Control testing program, documented under our ISO 9001:2008 registered Quality Management System.
96-well SPE plates are available in two types, the industry standard ISOLUTE-96 (fixed well plate) style, and the modular ISOLUTE Array design. 96-well plates are generally used to extract samples of up 1-2 mL in volume.
Use ISOLUTE® 96 fixed well plates (2 mL/well) for applications where high numbers of samples are routinely analysed. ISOLUTE-96 plates can be processed using vacuum or positive pressure manifold systems, and are compatible with popular automation systems.
ISOLUTE® Array modular plates (1 or 2 mL/well) can be ordered pre-assembled with 96 sorbent filled wells, or wells can be purchased separately, and base plates can be populated with as many or as few wells as required. Use ISOLUTE Array plates when developing a new method, or when sample numbers less than 96 are routinely analysed. Seal unused positions with sealing strips. Convert Array wells for use on luer fittings using adaptors.
ISOLUTE® SPE sorbents are available packed into columns ranging from 1 to 70 mL reservoir volume. They can be processed using vacuum or positive pressure, with both manual and automated systems.
All ISOLUTE® columns have industry standard Luer outlets, ensuring compatibility with vacuum manifolds and other processing systems.
For many ISOLUTE® columns, the same sorbent mass can be purchased in different sized columns. This provides flexibility in method devlopment and automation compatibility.
Tabless versions of the 1, 3 and 6 mL columns are available to meet specific automation system requirements.
Manufactured using high grade polypropylene, and fitted with polyethylene frits as standard, ISOLUTE® SPE columns have high levels of cleanliness, to prevent extract contamination. For higher sensitivity analysis, columns are available fitted with stainles steel frits (1,3 and 6 mL only).
Alternatively, selected sorbents are available packed in to glass columns (6 mL only) fitted with PTFE frits.
A wide range of sorbent chemistries is included in the ISOLUTE® SPE product range. In order to simplify the choice of SPE approach, ISOLUTE® sorbents are grouped into four main types, based on their retention mechanism:
In addition, a number of speciality and application specific phases are available.
ISOLUTE sorbents are available packed into a range of columns and plate formats. 96-well plates come in two types, the industry standard ISOLUTE-96 (fixed well plate) style, and the modular ISOLUTE Array design.
SPE columns are available with reservoir volumes ranging from 1 mL to 70 mL, with tabless options for automation compatibility, and options for high sensitivity analyses.
ISOLUTE SPE products are manufactured to the highest quality standards. State of the art manufacturing techniques are supported by a comprehensive Quality Control testing program, documented under our ISO 9001:2008 registered Quality Management System.
ISOLUTE® non-polar sorbents provide hydrophobic (reversed phase) interactions and are usually used to extract a range of analytes with some hydrophobic character from aqueous sample matrices. They sorbents range from the highly retentive ISOLUTE ENV+ through to the less retentive C2. Choose highly retentive sporbents to extract more polar analytes, and less retentive phases to extract more hydrophobic analytes.
Silica based non-polar sorbents are available in both non-endcapped* and, in certain cases, endcapped versions, to meet the needs of particular applications. Non-end capped sorbents are particularly useful when utilizing secondary silanol interactions for extraction of basic compounds.
*Endcapping is a chemical process used to reduce the concentration of silanol groups on the surface of bonded silica particles. Silanol groups povide polar and weak cation echange secondary interactions, which can be expolited to increase recovery and extract cleanliness for basic analytes.
Use mixed-mode sorbents to extrct acidic or basic analytes from complex matrices such as biological fluids using a dual retention mechanism. Mixed-mode sorbents provide two primary retention mechanisms: non-polar (hydrophobic) and anion exchange (anion or cation). The dual retention mechansims allow the use of rigorous interference elution regimes, providing extremely clean extracts and reducing matrix effects in LC-MS/MS analysis.
Isolate ionizable (acidic or basic) analytes from aqueous or partially aqueous samples using ion exchange retention mechanisms.
Cation exchange sorbents contain acidic groups that can be used to retain basic analytes. Stong cation exchange sorbents (such as ISOLUTE® SCX-2 and SCX-3) contain sulfonic acid groups that maintain a permanent negative charge under all pH conditions.
Weak cation exchange sorbents (such as ISOLUTE® CBA) contain carboxylic acid groups that have a negative charge under some pH conditions, and are neutralized at low pH.
Anion exchange sorbents contain basic groups that can be used to retain acidic analytes. Stong anion exchange sorbents (such as ISOLUTE® SAX and PE-AX) contain quaternary amine groups that maintain a permanent positive charge under all pH conditions.
Weak anion exchange sorbents (such as ISOLUTE® NH2 and PSA) contain primary or secondary amine groups that have a positive charge charge under some pH conditions, and are neutralized at low pH.
Polar SPE sorbents are used to isolate polar analytes or remove interferences from non-polar sample matrices using polar retention mechanisms (e.g. hydrogen bonding or dipole-dipole interactions). Note that some sorbents (ISOLUTE® CN, NH2 and PSA) can also be used in other retention modes.
See individual product pages for more information.
Extraction of THC using EVOLUTE® HYDRO with in-well hydrolysis.
ISOLUTE® SLE+ Supported Liquid Extraction plates and columns from Biotage is the trusted SLE product with more than 15 million run samples and hundreds of proven applications with global support. Product manager Paul Roberts gives you the quick overview in this video.
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Applications chemist Zoe Grosser talks about why SPE is becoming the preferred sample preparation technique in environmental labs. From NEMC 2018.
Biotage presented five new posters at the ASMS conference in San Diego this June. We've collected them all below for download. P177: Comparison of Sample Preparation Options for the Extr...
08-13-2018The Biotage 2018 catalog of analytical sample preparation products for chemistry professionals is now available. This edition includes a comprehensive range of sample preparation consumables for bi...
01-11-2018On July 1st, Biotage expanded its direct sales organization and opened the door to analytical customers in Italy. Account Manager Gianluca Pessoni is added to the Italian team, filling the ana...
07-17-2017This application note from Biotage Labs describes a method for extracting nitrosamines from urine. The ISOLUTE® SLE+ sample preparation method allows for a fast extraction with sufficient sampl...
06-28-2017This application note describes the extraction of amphetamine and amphetamine-style compounds from hair matrix prior to GC/MS analysis following bead homogenization. This application note describe...
06-15-2017This application note describes the operating conditions for the automated fractionation of EPH into aliphatic and PAH fractions using the ISOLUTE® EPH column in conjunction with the Biotage&re...
03-20-2017This application note describes the extraction of 49 drugs of abuse from whole blood, prior to UPLC-MS/MS analysis. An effective and efficient ISOLUTE SLE+ protocol optimized the for ...
02-20-2017Now available, the Biotage 2016 catalog of analytical sample preparation products for chemistry professionals. This edition includes a comprehensive range of products for bioanalysis, forensic, cli...
01-05-2016Dilute and shoot (D&S) methods are promoted for use by analytical instrument vendors in clinical, forensic toxicology and bioanalytical laboratories. More sensitive LC-MS/MS systems make this s...
08-11-2015Biotage, pioneers in flash chromatography, started packing columns with silica for flash purification 30 years ago. Some of the first employees are still with us today. In this video, site director...
03-27-2015Now available, the Biotage 2015 catalog of analytical sample preparation products for chemistry professionals. This edition includes a comprehensive range of products for bioanalysis, forensic, cli...
01-08-2015Buy your Biotage consumables through our website and shipping is free For a limited time, free shipping is applied to Biotage consumables orders through the Biotage website*. Let Biotage make orde...
07-29-2014Q:
What is the maximum capacity of an SPE column?
A:
The capacity of a sorbent is defined as the total mass of strongly retained analyte that can be retained by a given mass of the sorbent under optimum conditions.
For most sorbents, this can range from 1-5% by weight. 100 mg of sorbent might retain 1-5 mg of analyte.
Ion exchange sorbents typically have a capacity of 0.3-1 meq/g.
07-01-2013 Go to the Q&A Main Section >ISOLUTE® ENV+ is a highly retentive non-polar SPE phase, for the extraction of polar analytes that are not retained by C18 or C8 sorbents.
One product with multiple applications, simplifying and streamlining your mycotoxin analysis procedures.
ISOLUTE® QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) products provide simple clean-up of complex samples using salt assisted extraction and partitioning, followed by dispersive SPE.