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Two New Methods for Extraction of a Steroid Hormone Panel from Human Urine

27 September 2019

Two new application notes from Biotage describe both manual and automated procedures for sample pre-treatment and extraction of a panel of 19 steroid hormones from human urine, using EVOLUTE® EXPRESS ABN (Application Note Number AN922) or ISOLUTE® SLE+ supported liquid extraction (Application Note Number AN923). Manual sample preparation is performed using Biotage® Pressure+ 96 Positive Pressure Manifold and the automated method uses Biotage® Extrahera™.


Application Note Number AN922

EVOLUTE® EXPRESS products dramatically improve flow characteristics and enhance sample preparation productivity. 

Download Application Note AN922 


Application Note Number AN923

ISOLUTE®  SLE+ Supported Liquid Extraction products offer an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation, and significantly reduced sample preparation.

Download Application Note AN923 



  • Cortisol
  • 18-OH-Corticosterone
  • 21-Deoxycortisol
  • Cortisone
  • Estradiol
  • 17-OH-Pregnenolone
  • Aldosterone
  • 11-Deoxycortisol
  • Corticosterone
  • Estrone
  • Dehydroepiandrosterone (DHEA)
  • 17-OH-Progesterone
  • Dehydroepiandrosterone sulfate (DHEAS)
  • Testosterone
  • Dihydrotestosterone (DHT)
  • Pregnenolone
  • Androstenedione
  • 11-deoxycorticosterone
  • Progesterone


Featured Videos

Biotage® PRESSURE+ 96 Manifold Demonstration

ISOLUTE® SLE+ Supported Liquid Extraction


Biotage® Extrahera™ Presentation