Organic and medicinal chemists frequently utilize flash chromatography to purify their reaction mixtures. Normal-phase flash chromatography is most often used but may not the best methodology, especially when the compounds are quite polar and/or ionizable.
For these molecules, reversed-phase flash chromatography is preferred but often is not used due to an uncertainty regarding the best solvent choices and the reversed-phase mechanism. In this post, I will discuss how organic solvent choice in reversed-phase chromatography can influence the chromatographic separation.
Continue reading How does mobile phase organic solvent choice impact reversed-phase flash column chromatography?
Have you ever run flash column chromatography with mass detection (Flash-MS) and observed the total ion current or TIC increase during the purification only to find that there was no discernible compound contributing to the effect?
In this post I discuss how I came across this issue and the solution I found to work.
Continue reading How do I remove an annoying MS TIC background?