Increasingly, organic and medicinal chemistry labs use mass-directed flash chromatography to isolate synthesized compounds. Mass-directed flash chromatography benefits are many, including collecting only the targeted molecule(s) in the reaction mixture. This approach simplifies compound purification since you know what you have made and it’s associated mass.
However, there are mass detection nuances that can be challenging. One of these is to know when an acid should be added to the mass detector’s make-up solvent to protonate targeted molecules. In this post, I will provide some insight on this topic.
Continue reading When should I add acid to my detector make-up solvent when using mass-directed flash chromatography?
In this post, I will look at the impact modifying mobile phase pH can have on reversed-phase separations.
Continue reading Using pH to optimize reversed-phase flash chromatography separations