The answer to this question is yes, reversed-phase can sometimes provide a better separation and thus better purification than normal-phase. When is reversed-phase likely to be the better choice is a different, and likely better, question.
In this post I will try to demonstrate when reversed-phase is likely the better purification mode.
Continue reading Can reversed-phase flash chromatography purify better than normal-phase?
For chemists, flash chromatography is part of their everyday synthesis workflow. For most syntheses, crude reaction mixtures are purified by normal-phase (aka adsorption) chromatography. There are times; however, where the crude mixture’s complexity and polarity make normal-phase chromatography very challenging. For these situations, reversed-phase (aka partition) chromatography may be a preferred option.
But, if you have only one flash system available, can you, should you, and how do you efficiently switch from non-polar, normal-phase solvents to polar, reversed-phase solvents – and back again without issues? In this post I’ll attempt to shed some light on the topic.
Continue reading How can I perform normal-phase and reversed-phase column chromatography on one flash system?