How does solvent choice impact reversed-phase flash chromatography separations?

I have recently posted on how solvent choice influences the separation of hard to resolve compounds using normal-phase flash chromatography. As a chemist with an inquiring mind, I thought I would expand my research beyond normal-phase and see what happens in reversed-phase.

In this post, I share my results. 

Continue reading How does solvent choice impact reversed-phase flash chromatography separations?

How can I modify my flash chromatography method to separate chemically similar compounds?

The challenges organic, medicinal, and natural product chemists face are many: from designing reactions, to optimizing synthesis, work-up / extraction, and purification / isolation of the desired compound or compounds. Among those issues related to purification / isolation is the common problem of separating compounds with similar chemistry that either co-elute or separate poorly.

In this post I will discuss some tips on how to “resolve” this issue (yes, pun intended).

Continue reading How can I modify my flash chromatography method to separate chemically similar compounds?

What do I do if a 2-solvent gradient will not separate my sample?

Usually, a 2-solvent or binary gradient will separate your desired compound from the by-products and impurities. Sometimes though, you can encounter a mixture in which some compounds co-elute and are not separable with any binary gradient you try.

I encountered this situation recently while trying to purify a lavender essential oil and have dedicated this post to how I solved the problem.  Continue reading What do I do if a 2-solvent gradient will not separate my sample?