So, what exactly is a column volume in flash column chromatography and how is it determined?

In many of my previous posts I have used the term column volume, typically abbreviated as CV, as a value used to help determine separation quality and loading capacity.  However, I recently was asked a question about this topic from a chemist who understands the column volume concept but wanted to better understand its definition and how it is determined.

In this post I will explain what a column volume is and how it is determined empirically.

 

Read More Here!

Published by

Bob Bickler

Technical Specialist, Biotage

2 thoughts on “So, what exactly is a column volume in flash column chromatography and how is it determined?”

Leave a Reply to abott Cancel reply

Your email address will not be published. Required fields are marked *