Setting the Right Flow Rate for Flash Column Chromatography

How does flow rate impact my flash column chromatography separation?  This is the kind of question I frequently get.  After all, we all know that high flow rates that are too high or too low mean bad prep HPLC chromatography.  Well, this is not necessarily true in flash chromatography.

In chromatography there are three inter-related variables which impact your separation and are represented on the  chromatographer’s triangle.

Chromatographer's triangle 2.jpgIn practice only two of these variables can be optimized.  Increasing flow rate decreases either load (if you need to maintain a purity level) or purity (if you need to maintain a throughput) because of increase sample dispersion and band-broadening.  If you increase load, you need to decrease the optimal flow rate or purity will suffer.  You get the idea.

In this post I will discuss the impact that flow rate has on flash chromatographic performance.

Flash column chromatography is a vital part of the organic or natural product chemist’s tool box.  An organic or medicinal chemist may only need a target intermediate compound at 80% purity, while a natural product chemist may need better than 95% purity.

In previous posts I have talked about flow rate and linear velocity and how it is important to maintain linear velocity when scaling up a purification. Until now, though, I have not discussed the impact flow rate has on linear velocity and, likewise, load and purity.

Students of chromatography know about the van Deemter curve and how it models the correlation between column efficiency (plate count) and flow linear velocity. For every particle size media used for a stationary phase there is a unique, optimal linear velocity.  Generally, smaller media particles,  have higher optimal flow rates.  Additionally, the range of optimal flow rates is increased as well.  When the flow rate is optimized so is the loading and purity due to better separation between compounds in the mix. However, the optimal flow rate for a cartridge typically translates to a low flow rate thus a longer purification time.

chromatographers-triangle

Trade-off decision time. Ask yourself, what is more important – fast purification with high yield or a high purity product with high yield? So, if you want to purify your sample as fast as possible with maximum purity and yield (the ideal situation) you will need to increase cartridge size because your purification will be load limited (but that increases purification costs). But if purity is the priority, then use a flow rate that gives the optimal linear velocity for any specific flash cartridge.

So what is “optimal” when it comes to flow rates on cartridges packed with different particle size media? Well, there is published data1, primarily for analytical HPLC columns, which shows the optimal linear velocity is about ~0.8 mm/sec for a 10 micron media and ~1.8 mm/sec for a 5 micron particle. Bigger particles such as those used in flash and column chromatography demand even slower linear velocity to be optimal. However, because of these slower than ideal flow rates flash cartridge manufacturers have moved to smaller, more efficient 25 µm flash media in recent years. After all, flash chromatography is supposed to be fast, right?

So, what do these linear velocities translate to in terms of flow rate? Well, if we assume a 50 µm particle has an optimal velocity of 0.1 mm/sec and a 25 micron has an optimal velocity of 0.3 mm/sec then your flow rates are ridiculously slow, much too impractical for routine use. How slow? See the Table 1 below…

Table 1. Effective flow rates for various cartridge sizes at optimal linear velocity. Flow rate data for Biotage® SNAP and SNAP Ultra cartridges.

Column size (g) 50 µm media 25 µm media
10 2 mL/min 6 mL/min
25 3 mL/min 12 mL/min
50 6 mL/min 21 mL/min
100 6 mL/min 21 mL/min
340 20 mL/min 70 mL/min

Ouch, really low flow rates. So then, what is a reasonable and practical linear velocity for flash-grade media that will provide sufficient loading capacity, separation performance (purity), and purification time?

Well, most flash cartridge manufacturers have developed and published flow rate ranges they believe are practical. For flash chromatography, as with HPLC, the optimal flow rate is particle size dependent.

To study the impact of flow rate on separation performance I performed a test where I separated a mix of two compounds, ethyl and propyl paraben, by reversed-phase flash chromatography at four flow rates from 20 to 50 mL/min in 10 mL/min increments.

I used two different cartridges, a 12 gram Biotage® SNAP KP-C18-HS with a nominal 50 µm particle media and a 12 gram Biotage® SNAP Ultra C18 packed with a 25 µm media. To eliminate gradient effects, I performed the separation using an isocratic 50:50 methanol/water mobile phase and maintained the same sample load, 50 mg per run.

The results obtained showed a pronounced separation (resolution) decline with increasing flow rate with the larger 50 µm particle media, Figure 1. The smaller 25 µm particle media showed separation stability from 20 through 40 mL/min with only a slight decline at 50 mL/min, Figure 2.

Separations obtained of a 2-component mix by a reversed-phase 12 gram Biotage KP-C18-HS cartridge at 20, 30, 40, and 50 mL/min show a maximum separation at 20 mL/min as indicated by its nearly baseline separation. With increasing flow there is decreasing separation.
Figure 1. Separations obtained of a 2-component paraben mix by a reversed-phase, 50 micron, 12 gram, Biotage KP-C18-HS cartridge at 20, 30, 40, and 50 mL/min (top to bottom) show a maximum separation at 20 mL/min as indicated by its nearly baseline separation between peaks 2 and 3.
An isocratic separation of two compounds by reversed-phase using a 25 um SNAP Ultra C18 at 20, 30, 40, and 50 mL/min shows minimal separation decline with increasing flow rate.
Figure 2. Isocratic separations of two parabens by reversed-phase using a 25 um, 12 gram SNAP Ultra C18 cartridge at 20, 30, 40, and 50 mL/min (top to bottom) show minimal separation decline with increasing flow rate.  At 50 mL/min there is some minimal degradation.

So, this data says that setting the correct linear velocity is more important with larger particle media than with smaller media and that there is flow rate flexibility in flash chromatography, especially when you use smaller particle cartridges.

Have you noticed anything different?  If so, share your results.

HPLC to UPLC Method Migration: An overview of Key Considerations and Available Tools. Swartz, Michael, Waters Corp., presented at PittCon 2007.

Published by

Bob Bickler

Technical Specialist, Biotage

Leave a Reply

Your email address will not be published. Required fields are marked *