All there is to know about flash purification – a blog by Biotage
Purifying ionic compounds by flash column chromatography
One of the more challenging purifications is that of water-soluble, ionizable compounds. Typically, normal-phase with silica is not used because of the probable non-reversible interactions, especially between the ionized amines interacting and the ionizable silanols. With normal-phase out of the purification solution that leaves ion exchange and reversed-phase as chromatographic options.
In this post I will discuss the use of reversed-phase and the influence pH and buffers have on the chromatography of some ionic, water soluble compounds.