How to choose between normal- and reversed-phase flash column chromatography is an excellent question and one that my readers often ask. Those who use column chromatography know that as long as the reaction products or compounds are fairly non-polar and near neutral pH they will have successful purifications. However, when your mixture’s chemical characteristics are more challenging (polar, non-polar, basic, acidic) there are other options that are available to successfully separate pure compounds.
In this posting I will discuss the criteria you can use to guide your choice between normal- or reversed-phase flash chromatography.
In a previous post I shared results of experiments where I evaluated selected organic solvents for sample dissolution and injection for reversed-phase flash purification. I demonstrated that DMF and DMSO both are excellent solvents for this purpose and actually provide better chromatography than methanol, acetonitrile, and acetone.
In this post I report some surprising results from follow-on work evaluating the impact of increased injection volume using DMF and DMSO as the sample diluent/injection solvent.
In previous posts I have touched upon various sample loading options and how they impact flash chromatographic performance, primarily in normal-phase flash purification. As the use of reversed-phase flash chromatography has steadily increased over the past few years I thought it would be a good idea to discuss one of the most important factors impacting its success.
In this post I discuss the results of some of my original research studying the impact of injection solvent choice on reversed-phase flash separations. Continue reading Which injection solvents should I use for reversed-phase flash purification?
Getting the most benefit from your crude sample purification with column chromatography or flash chromatography involves optimizing many variables. In previous posts I have talked about selecting the best solvents, their ratios, and maximizing load based on TLC Rf data. These are all important chromatography-generated variables but now I would like to share some tips on actual technique differences and their impact on purification performance.
In particular in this post I will focus on the benefits and drawbacks of liquid loading and dry loading. Both have their place in liquid chromatography but when should one technique be used over another?
Here is a video post that is somewhat of the commercial nature but I think it could be interesting for you to see some different loading options one can use with Biotage® ZIP and SNAP Ultra flash chromatography columns.
Dr. Greg Saunders goes through techniques for loading your Biotage flash purification columns. The versatile Biotage® SNAP Ultra cartridges gives you the possibility to load your sample in up to seven different ways. Read more about our flash consumables at biotage.com/product-group/flash-cartridges
Feel free to share the video and write me a comment of what you think about it here below.