The UV absorption spectrum of some solvents overlaps with the product they dissolve, meaning that fraction collection processes cannot distinguish between solvent and product. The phenomenon, especially when applied to mobile phase gradients, can also result in drifting baselines and, in a worst case, swamp the sample out completely. Luckily, there is technology that solves this problem.
Here we go – the first step towards the ultimate flash purification online resource. Hope to see you soon!