Applying green chemistry principals to flash purification is becoming increasingly important. In this post, I discuss ways to make flash column chromatography greener by reducing solvent use through optimization of gradient shape.
This is a follow-on to my earlier post where I presented some greener alternatives to DCM as a solvent in flash column chromatography.
Flash column chromatography is used by between 20 and 40 thousand organic synthesis chemists worldwide, an amazing number. For most of these chemists flash chromatography is an important part of their daily workflow but allocating time for good method development is often not considered, which can lead to less than ideal purification results.
In this post I focus on how allocating just 10 minutes on thin-layer chromatography (TLC) for method development can save you a lot of grief later on.
The term “Green Chemistry” has become a major part of the science community’s lexicon. When I think about green chemistry and its relationship to flash column chromatography I think of two specific areas where it applies…
1. Replacing chlorinated solvents with those considered more environmentally friendly
2. Reducing solvent use and waste generation with more thoughtfully applied chromatography principals
In this post I will discuss alternatives to chlorinated solvent-based flash column purification. In the future, look for a post on reducing solvent use (and its waste).
For many chemists performing bench-scale organic synthesis flash column chromatography is the primary purification technique. When your synthesis needs scaling to multi-gram levels, so does the flash purification. The logical approach is to just increase the flash cartridge or column size, but this is only part of the solution.
In this post I discuss the process of simplified flash purification scale-up.
How to choose between normal- and reversed-phase flash column chromatography is an excellent question and one that my readers often ask. Those who use column chromatography know that as long as the reaction products or compounds are fairly non-polar and near neutral pH they will have successful purifications. However, when your mixture’s chemical characteristics are more challenging (polar, non-polar, basic, acidic) there are other options that are available to successfully separate pure compounds.
In this posting I will discuss the criteria you can use to guide your choice between normal- or reversed-phase flash chromatography.
In a previous post I shared results of experiments where I evaluated selected organic solvents for sample dissolution and injection for reversed-phase flash purification. I demonstrated that DMF and DMSO both are excellent solvents for this purpose and actually provide better chromatography than methanol, acetonitrile, and acetone.
In this post I report some surprising results from follow-on work evaluating the impact of increased injection volume using DMF and DMSO as the sample diluent/injection solvent.
In previous posts I have touched upon various sample loading options and how they impact flash chromatographic performance, primarily in normal-phase flash purification. As the use of reversed-phase flash chromatography has steadily increased over the past few years I thought it would be a good idea to discuss one of the most important factors impacting its success.
In this post I discuss the results of some of my original research studying the impact of injection solvent choice on reversed-phase flash separations. Continue reading Which injection solvents should I use for reversed-phase flash purification?
Getting the most benefit from your crude sample purification with column chromatography or flash chromatography involves optimizing many variables. In previous posts I have talked about selecting the best solvents, their ratios, and maximizing load based on TLC Rf data. These are all important chromatography-generated variables but now I would like to share some tips on actual technique differences and their impact on purification performance.
In particular in this post I will focus on the benefits and drawbacks of liquid loading and dry loading. Both have their place in liquid chromatography but when should one technique be used over another?
Here is a video post that is somewhat of the commercial nature but I think it could be interesting for you to see some different loading options one can use with Biotage® ZIP and SNAP Ultra flash chromatography columns.
Dr. Greg Saunders goes through techniques for loading your Biotage flash purification columns. The versatile Biotage® SNAP Ultra cartridges gives you the possibility to load your sample in up to seven different ways. Read more about our flash consumables at biotage.com/product-group/flash-cartridges
Feel free to share the video and write me a comment of what you think about it here below.