Up to six compounds can be easily separated with an automated step-gradient optimizer embedded in modern flash chromatography systems.
For many chemists lab budgets, especially for consumable items, are limited. One way of trying to stretch their lab budget is to reuse disposable flash chromatography cartridges.
In this post I will show how regardless of the cartridge brand used, repeated use of silica flash cartridges results in loss of compound resolution and fraction purity.
Got a Biotage® SNAP Ultra cartridge but no Isolera™ flash system? Don’t worry, the design of Biotage SNAP cartridges allows use on virtually any modern system for flash purification.
There is a flash system on the market with built-in mass detection, called Isolera Dalton. It uses pretty cool technology to produce its final mass spectrum, compiling data from hundreds of individual mass spectra in real time.
This article touches on the latest development in purification, ACI™ Accelerated Chromatographic Isolation, and how future needs of the typical Flash user will be met; way past the current limits of traditional flash. Flash evolved.
Every chemist has been taught how to use and deal with glass columns in schools, so they have fundamental background experience. But unlike traditional normal phase flash, many chemists have little experience of dealing with reversed phase, and especially reversed phase flash. Continue reading Scaling up reversed Phase Flash Purification
This article discusses two recent developments in flash purification that have increased cross-over with traditional HPLC techniques: cartridge media properties and new detectors in flash systems.
The UV absorption spectrum of some solvents overlaps with the product they dissolve, meaning that fraction collection processes cannot distinguish between solvent and product. The phenomenon, especially when applied to mobile phase gradients, can also result in drifting baselines and, in a worst case, swamp the sample out completely. Luckily, there is technology that solves this problem.
Here we go – the first step towards the ultimate flash purification online resource. Hope to see you soon!