In all my years of working with medicinal and organic chemists, I have found that choosing how many grams of silica to use for purification by flash chromatography is something frequently guessed at. Getting the size of the column right is awfully important because using too few grams of silica will doom your purification to failure and using more an optimal mass of the stationary phase means the purification consumes excess silica, solvents, and a chemist’s time. To determine the optimal amount of silica for a purification, I rely on a factor called ΔCV (delta Column Volume) to identify the best loading capacity on any cartridge. I have also found that ΔCV this is a better loading capacity predictor for flash purification than ΔRf.
Continue reading How do I Choose the Right Column Size for Purification by Flash Chromatography?
Up to six compounds can be easily separated with an automated step-gradient optimizer embedded in modern flash chromatography systems.
Continue reading The Step Gradient Explained
For many chemists lab budgets, especially for consumable items, are limited. One way of trying to stretch their lab budget is to reuse disposable flash chromatography cartridges.
In this post I will show how regardless of the cartridge brand used, repeated use of silica flash cartridges results in loss of compound resolution and fraction purity.
Continue reading Why Reusing Flash Cartridges is Bad Science
Got a Biotage® SNAP Ultra cartridge but no Isolera™ flash system? Don’t worry, the design of Biotage SNAP cartridges allows use on virtually any modern system for flash purification.
How to use SNAP Ultra on CombiFlash Rf Systems
There is a flash system on the market with built-in mass detection, called Isolera Dalton. It uses pretty cool technology to produce its final mass spectrum, compiling data from hundreds of individual mass spectra in real time.
Continue reading How is a Mass Spectrum Actually Generated?
This article touches on the latest development in purification, ACI™ Accelerated Chromatographic Isolation, and how future needs of the typical Flash user will be met; way past the current limits of traditional flash. Flash evolved.
Continue reading Find Out Why The Era of Regular Flash Purification Has Come To An End
This article discusses two recent developments in flash purification that have increased cross-over with traditional HPLC techniques: cartridge media properties and new detectors in flash systems.
Continue reading The Lines Between Purification and Analysis are Blurring…