Using TLC to Scout Flash Chromatography Solvents

TLC is the tool most used for normal-phase flash chromatography method development. For many chemists, a solvent system of hexane (or heptane) + ethyl acetate is the first, and sometimes only, solvent system evaluated. Though often useful, ethyl acetate may not always provide the optimal purification conditions. Continue reading Using TLC to Scout Flash Chromatography Solvents

Why Reusing Flash Cartridges is Bad Science

For many chemists lab budgets, especially for consumable items, are limited.  One way of trying to stretch their lab budget is to reuse disposable flash chromatography cartridges.

In this post I will show how regardless of the cartridge brand used, repeated use of silica flash cartridges results in loss of compound resolution and fraction purity.

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Find Out Why The Era of Regular Flash Purification Has Come To An End

This article touches on the latest development in purification, ACI™ Accelerated Chromatographic Isolation, and how future needs of the typical Flash user will be met; way past the current limits of traditional flash. Flash evolved.

Continue reading Find Out Why The Era of Regular Flash Purification Has Come To An End

Scaling up reversed Phase Flash Purification

Every chemist has been taught how to use and deal with glass columns in schools, so they have fundamental background experience. But unlike traditional normal phase flash, many chemists have little experience of dealing with reversed phase, and especially reversed phase flash. Continue reading Scaling up reversed Phase Flash Purification

The Lines Between Purification and Analysis are Blurring…

This article discusses two recent developments in flash purification that have increased cross-over with traditional HPLC techniques: cartridge media properties and new detectors in flash systems.

Continue reading The Lines Between Purification and Analysis are Blurring…

Overcoming UV-Absorbing Mobile Phases

The UV absorption spectrum of some solvents overlaps with the product they dissolve, meaning that fraction collection processes cannot distinguish between solvent and product. The phenomenon, especially when applied to mobile phase gradients, can also result in drifting baselines and, in a worst case, swamp the sample out completely. Luckily, there is technology that solves this problem.

Continue reading Overcoming UV-Absorbing Mobile Phases