All there is to know about flash purification – a blog by Biotage
Flash column equilibration – is it a waste of time or necessary step?
Flash column chromatography has been practiced by chemists since the 1970s. That practice required the silica in the column be properly wetted to remove trapped gasses to ensure uniform flow (remember those days of not letting air into the column?). Today, with automated flash chromatography systems and pre-packed cartridges as the norm, chemists ask me – do I really need to pre-equilibrate my cartridge?
In this post I explore the impact on chromatography that equilibration, and lack thereof, has on separation performance.