All there is to know about flash purification – a blog by Biotage
Flash column chromatography equilibration speed – how fast can you go?
Equilibrating silica flash chromatography columns is something I always do. There are chemists who see this as an unnecessary, time-and-solvent-wasting step. Because getting consistent, predictable results is a priority, I equilibrate to remove the variability that can be caused by heat generated as solvent initially contacts the silica. Consistency is really important when running flash column chromatography because re-runs are time consuming and may put your compound at risk.
In this post, I examine the role of equilibration speed and duration to show its impact, or lack there of, on purification performance.