Does particle shape and/or particle size impact flash column chromatography load limits?

There are many factors which influence successful flash column chromatography. One of those factors is sample load which itself is influenced by things like selectivity, efficiency, dissolution solvent, and load technique. Several of these factors I have addressed in previous posts. Of these, selectivity and efficiency are specific to a media’s physical and chemical characteristics.

In this post I will show if particle size and/or particle shape can influence loading capacity.  Additionally, I will show the positive impact that surface area has on flash column chromatography purification.

In the beginning times of flash chromatography there was only irregular (aka granular) silica and chemists around the world were generally pleased with the results.  W. Clark Still, in his seminal paper on flash chromatography (1978), found that 40-63 µm silica with 60Å pores worked best for his purification needs.  As flash chromatography became more popular in the labs of organic and medicinal chemists, entrepreneurial companies started commercial production of prefilled glass columns and disposable polypropylene cartridges with the same particle size silica.

Over time, however, the need arose for silica with better separating power and increased loading capacity.  To address this need, disposable cartridges with smaller particles of the same or similar silica became available.  This change did increase the separating power and loading capacity compared to the original 40-63 µm (Figures 1 and 2) but at the cost of higher backpressure when operating at the same flow rate and with the same solvents.

Separation of 2 grams of a 5-component sample on a granular, 40-64 μm silica cartridge (50 gram size) show an incomplete separation of the last three compounds.
Figure 1.  Separation of 2 grams of a 5-component mixture on a granular, 40-63 μm silica cartridge (50 gram size) shows an incomplete separation of the last three compounds.
High-performance granular silica cartridge (50 gram size) purification of 2 grams of a 5-component sample shows nearly baseline separation.
Figure 2. High-performance (15-40 μm) granular silica cartridge (50 gram size) purification of 2 grams of a 5-component mixture shows nearly baseline separation.

In the chromatograms above, a “standard” 40-63 µm granular silica-filled 50 gram cartridge and a cartridge packed with 50 grams of a finer “high-performance” granular 15-40 µm silica were used to separate 2 grams of a multi-component mixture.  Cartridge dimensions, mobile phase gradient, and flow rates were identical and the 15-40 µm cartridge did provide an improved separation and, therefore, an inferred increase in loading capacity.

A halving of particle size doubles the number of theoretical plates (e.g. efficiency) but that alone will only increase compound resolution by 41% and it is resolution that dictates loading capacity.  A halving of particle size will also increase flow resistance or back pressure ~4 times compared to the larger particle media as shown in Figure 3.

Pressure vs. flow graph for granular 40-63 μm and 15-40 μm silica in 25 gram cartridges.
Figure 3. Pressure vs. flow graph for granular 40-63 μm and granular 15-40 μm silica in 25 gram cartridges shows a considerably larger back pressure for the smaller particle media.

Resolution is influenced by three factors – the cartridge efficiency (plates or band width), compound retention, and compound selectivity (relative spacing between adjacent peaks).  Plate count (efficiency), however, has just moderate impact on resolution.

As can be seen in the calculation for resolution (Rs) below the influence of efficiency (N) is based on its one fourth of its square root. Selectivity (α), however, is directly proportional to resolution so it has the biggest impact on resolution and therefore on loading capacity.

Rs = ¼*N1/2 *(α-1)*(k/(1+k))

Where:

N = efficiency

α = selectivity

k = capacity factor (retention)

So, in order to increase loading capacity you need to improve selectivity, which may be possible with a change in elution solvents or stationary phase.  If that is not possible then improve separation efficiency by moving to a smaller particle media.

There is a way chemists can enjoy the benefits of faster purification (without sacrificing purity);  when spherical silica particles are used.  Spherical shaped particles pack more uniformly into the cartridges which can slightly improve peak shape and resolution while generating lower backpressure.

Granular silica cartridges often have “pockets” of dead space where there is no silica. These pockets allow solvent and sample components to become trapped and pool which causes bandspreading and reduced resolution (eddy effect).  Generally though, the physical shape of the particle influences pressure more than it does the actual chromatography, Figure 4.

Pressure vs. flow graph for granular and spherical silicas. Spherical silica typically provides lower pressures than granular silica.
Figure 4. Pressure vs. flow graph for granular and spherical silica. Spherical silica typically provides lower pressures than granular silica, especially with smaller diameter particles.

To help show the performance differences, or lack there of, between granular and spherical silica, two different commercially available 12 gram high-performance flash cartridges, one with granular silica and the other with spherical silica, were used with the same elution method to separate 200 mg of a 5-component mix, Figure 5. Because the media and cartridges are from different vendors, compound retention is different but the ability to separate the compounds, especially the last three, has not been influenced by particle shape.

A comparison of a 12 gram granular 15-40 um silica cartridge and a 12 gram spherical 20-40 um silica for the separation of a 5-component mix. Sample load was 200 mg and flow rate was 30 mL/min. The data show very similar separation performance though there is less compound retention with the spherical silica.
Figure 5. A comparison of a 12 gram granular 15-40 μm silica cartridge (top) and a 12 gram spherical 20-40 μm silica for the separation of a 5-component mix. Sample load was 200 mg and flow rate was 30 mL/min. The data show very similar separation performance though there is less compound retention with the spherical silica.

It is the silica surface area that impacts load more so than its shape.  Figure 6 shows how an increase in surface area impacts sample load and the resulting chromatography.

Load comparison between a granular 40-63 um, 500 m2/g silica (Biotage KP-Sil) and a 60 um spherical silica with 750 m2/g of surface area (Biotage KP-Sphere). The data show that the spherical, high surface area KP-Sphere has twice the capacity of the traditional granular silica.
Figure 6. Load comparison between a granular 40-63 μm, 500 m2/g silica (Biotage® KP-Sil) and a 60 μm spherical silica with 750 m2/g of surface area (Biotage® KP-Sphere). The data show that the spherical, high surface area KP-Sphere has twice the capacity of the traditional granular silica.

In the data above, the spherical silica (Biotage® KP-Sphere) has a similar particle size (60 µm) as the granular silica (40-63 µm) but because its surface area is 50% greater (750 m2/g vs. 500 m2/g), it is able to accommodate a higher sample load without separation loss or increase in back pressure.

Smaller particle size silica with higher surface area can further improve resolution and therefore loading capacity, which I have discussed previously. In Figure 7 we see the impact of both reduced particle size and increased surface area on chromatography – sharper peak shape, more complete separation, and increased load capacity.

200 mg purification on a very high surface area, 25 µm 10 g Biotage® SNAP Ultra cartridge. The silica's increased surface area and smaller particle size silica enable a doubling of the sample size without any separation loss vs. the traditional silica
Figure 7. A 200 mg purification on a 750 m2/g surface area, 25 µm 10 g Biotage® SNAP Ultra cartridge. This silica’s increased surface area and smaller particle size provide a better separation with higher loading capacity than either of the 12 gram cartridges in Figure 5.

So, the bottom line is changing particle size will alter loading capacity but a switch from granular to spherical will only change back pressure. To obtain the best performance use a high surface area spherical silica and you will maximize load and minimize backpressure.

Have you evaluated spherical silica cartridges?  Share your experiences with us.

Published by

Bob Bickler

Technical Specialist, Biotage

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