Up to six compounds can be easily separated with an automated step-gradient optimizer embedded in modern flash chromatography systems.
Continue reading The Step Gradient Explained
For many chemists lab budgets, especially for consumable items, are limited. One way of trying to stretch their lab budget is to reuse disposable flash chromatography cartridges.
In this post I will show how regardless of the cartridge brand used, repeated use of silica flash cartridges results in loss of compound resolution and fraction purity.
Continue reading Why Reusing Flash Cartridges is Bad Science
There is a flash system on the market with built-in mass detection, called Isolera Dalton. It uses pretty cool technology to produce its final mass spectrum, compiling data from hundreds of individual mass spectra in real time.
Continue reading How is a Mass Spectrum Actually Generated?
The UV absorption spectrum of some solvents overlaps with the product they dissolve, meaning that fraction collection processes cannot distinguish between solvent and product. The phenomenon, especially when applied to mobile phase gradients, can also result in drifting baselines and, in a worst case, swamp the sample out completely. Luckily, there is technology that solves this problem.
Continue reading Overcoming UV-Absorbing Mobile Phases