In previous posts I have touched upon various sample loading options and how they impact flash chromatographic performance, primarily in normal-phase flash purification. As the use of reversed-phase flash chromatography has steadily increased over the past few years I thought it would be a good idea to discuss one of the most important factors impacting … Continue reading Which injection solvents should I use for reversed-phase flash purification?
With most chromatographic purifications, only two solvents are needed to adequately separate compounds from each other. Unfortunately, there are instances where the separation is either poor or cannot be accomplished with “normal” elution conditions such as those with ionic or very polar organic molecules. In this post I offer some solutions to this issue.
With reversed-phase flash column chromatography becoming increasingly popular for routine purification, understanding how to make the cartridges last (since they cost more) is important to know. In this post I will mention a few tips to prolong reversed-phase cartridge life.
Have you ever wondered if there was a faster and cheaper way to purify your peptides? My colleagues and I in the peptide community rely almost exclusively on reversed-phase HPLC for delivery of highly pure peptide products. However, this process is often very time consuming and requires expensive columns and solvents to be successful. Alternatively, peptide purification via … Continue reading Optimizing a mobile phase gradient for peptide purification using flash column chromatography
In past posts I discussed which solvents work best for sample loading in reversed-phase flash chromatography. Recently, I was asked to provide some insight as which solvents are best in normal-phase flash column chromatography. Liquid loading of samples onto chromatography columns is not always straight-forward. If your sample is dissolved in the mobile phase, that can work but may … Continue reading Which sample solvents work best with normal-phase flash column chromatography?
In a previous post I shared results of experiments where I evaluated selected organic solvents for sample dissolution and injection for reversed-phase flash purification. I demonstrated that DMF and DMSO both are excellent solvents for this purpose and actually provide better chromatography than methanol, acetonitrile, and acetone. In this post I report some surprising results from follow-on … Continue reading How much DMF or DMSO can I inject on my reversed-phase flash chromatography cartridge?
Silica is the most commonly used sorbent for flash column chromatography. When solvent is pumped through a column packed with dry silica you may notice it gets warm and sometimes down right hot! In this post I will attempt to explain why this phenomena occurs.
For some cannabis-based product developers reversed-phase chromatography has become the analytical tool of choice for determining the extract content profile as well as for purification of specific cannabinoid compounds. However, the extracts often contain many other compounds which reduce load capacity and purity of the product(s) of interest and then require even more extensive clean-up. In this … Continue reading Cannabis extract purification using orthogonal flash column chromatography
With this post I would like to introduce a new colleague, Beth Denton, who I recently convinced to try flash chromatography for purification of peptides. She has kindly agreed to share her new flash column chromatography experience here. So, now, here’s Beth. As a peptide chemist, I was trained to purify my peptides with reversed-phase HPLC, just as … Continue reading Peptide purification with flash column chromatography – a beginner’s experience
It is hard for me to believe but the flash purification blog is a year old. This started as an idea within the technical group here at Biotage to share knowledge of flash column chromatography that we have learned over 30-plus years. Though I was reticent at first, now after only a year its hard to … Continue reading Celebrating 1 year of the flash purification blog